Evaluation of the antimetastatic and anticancer activities of morin in HER2‑overexpressing breast cancer SK‑BR‑3 cells

Morin (2',3,4',5,7‑pentahydroxyflavone), a flavonoid isolated from members of the Moraceae family and leaves Cudranaia tricuspidata Buread, is well‑known natural substance with anti‑inflammatory, antioxidative, antimetastasis, anticancer effects. However, its activity has not been comprehensively investigated in human epidermal growth factor receptor 2 (HER2)‑overexpressing breast cancer cells. Here, we evaluated effects morin on metastasis cell viability HER‑2‑overexpressing SK‑BR‑3 Our results revealed that (150‑200 µM) prevented endothelial (EGF)‑induced metastatic potential suppressed migration MMP‑9 by inhibiting EGFR signaling pathway cells gelatin zymography, wound healing assay western blotting. Interestingly, morin‑induced reductions were found to be associated inhibition HER2/EGFR sulforhodamine B also induced phosphorylation H2A.X downregulated expression levels RAD51 survivin, which implied DNA damage this accumulated Western blot analysis fluorescent immunocytochemisty showed activated autophagy after 24 h treatment was maintained at 48 when activation apoptosis via PARP cleavage resulted caspase‑3 ‑7, release cytochrome c cytosol mitochondria. In addition, p38 JNK enhanced treatment, suggested participate death. Taken together, present investigation indicates powerful therapeutic candidate for HER2‑overexpressing because it suppresses pathway, induces death potential.